I do not think there is an "easy" method. Nevertheless here is one approach:

1. Is your compound(s) secreted or not? Start with a bio-assay, test the growth media before after fungi growth (to remove the fungi centrifuge and filtrate through 0.2um filter. Also do MeOH extraction of the talus itself as control.

2a. If it is in the in the liquid do two-phase extraction with Hexane, then Chloroform, EtOAc and BuOH (every time against the aqueous phase).

2b. If in the Talus, extract with MeOH, add 9 volumes of water, mix well, and do two-phase extarction as above.

3. In each case take the organic phase, dry it, resuspend in small volume of MeOH and place on a small sterile Whatman 3 paper disk (you can make your own with a one hole puncher). Let the paper disk dry from the solvent.

Smear your bio-assay bacteria on agar, apply the dried paper disk, incubate and look for hallows.

There are other approaches, google natural products isolation...

Yoram

First of all check for the nature of the compound i. e. whether it is extracellular/intracellular. This can be done through spot inoculation or co-inoculation studies.

1. If it is an extracellular secretion then the process is bit easiesr you can try with culture filtrate of different days culture.

2. If it is intracellular, then you have to follow a sonication method to degrade the cell in order to relesae the compounds.

3. you can use organic solvents for extraction of trhe compound.

4. testing can be followed by various methods.

with regards

I think that you have to classified the fungus if you have any important compound. Here a method to extract the metabolite:

J Nat Prod. 2009 Apr;72(4):626-31. doi: 10.1021/np8004997.

Bioactive metabolites from the endophytic fungus Stemphylium globuliferum isolated from Mentha pulegium.

Debbab A1, Aly AH, Edrada-Ebel R, Wray V, Müller WE, Totzke F, Zirrgiebel U, Schächtele C, Kubbutat MH, Lin WH, Mosaddak M, Hakiki A, Proksch P, Ebel R.