NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs.
We have optimised our isolate plugs for 19 hrs.
How do I synchronize it now?
One issue is we get 12-13 bands only (picture attached) after 24 hrs of run.
Whereas the literature picture shows almost 19 bands (48 hrs run) - https://www.neb.com/products/n0340-lambda-ladder-pfg-marker.
Is running for 48 hrs the only solution?
What is the shape and size of the NEB ladder plug you use (it is inla syringe with spherical shape)?