NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs. 

We have optimised our isolate plugs for 19 hrs. 

How do I synchronize it now? 

One issue is we get 12-13 bands only (picture attached) after 24 hrs of run. 

Whereas the literature picture shows almost 19 bands (48 hrs run) - https://www.neb.com/products/n0340-lambda-ladder-pfg-marker.

Is running for 48 hrs the only solution? 

What is the shape and size of the NEB ladder plug you use (it is inla syringe with spherical shape)?

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