PCR based typing methods such as- Ribotyping, RAPD etc....
For Enterobacteriaceae members I found ERIC-PCR to be quite good.
Many Institutes do not have facilities for PFGE and MLST.
Some Institutes, though they have PFGE facility, due to its cumbersome initial optimizing would have cornered the machine.
Marcelo Mora
Depend on the funds available and in the species you are interested. MLVA is the best one in my opinion (for some species like Staphilo or Pseudomonas) if you have limited funding or if you do not want to depend on expensive equipment. But of course if you have funding then you can go with more expensive methods such as MLST.
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