Let me know more details about its composition. How it works? And its application?
Hi Dewan,
Though your question is a broad one, I will briefly answer it.
Ischemia is the condition where blood flow is limited to a specific area of a tissue. To mimic the ischemic situation, an ischemic buffer is used in the cell culture. This buffer must meet following conditions which are observed under ischemia,
1. Low oxygen partial pressure/No oxygen
2. Low pH due to anaerobic respiration
3. Lack of nutrients, especially glucose
4. Higher ion concentrations
Below is an example for a general ischemic buffer. You can compare the concentrations of ions and the other components with the normal media or HBSS (Hanks' Balanced Salt Solution)
137 mM NaCl, 15.8 mM KCl, 0.49 mM MgCl2, 0.9 mM CaCl2, 4 mM HEPES, 10 mM 2 deoxyglucose, 20 mM sodium lactate, and 1 mM sodium dithionite. pH 6.3 (Sun, Fan et al. 2015)
To simulate the ischemic situations in the cell cultures, we use this buffer coupled with zero oxygen partial pressure (use 95% Nitrogen and 5% CO2 instead of the normal cell culture atmosphere). The cells will be supplemented with the normal media after the ischemic period to mimic the re-perfusion.
Hope this would help you
Good Luck..!
Reference
Sun, L., H. Fan, L. Yang, L. Shi and Y. Liu (2015). "Tyrosol prevents ischemia/reperfusion-induced cardiac injury in H9c2 cells: involvement of ROS, Hsp70, JNK and ERK, and apoptosis." Molecules 20(3): 3758-3775.
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