Why DMSO(Dimethyl sulfoxide) is used in (PCR) polymerase chain reaction?
Hi Dewan
DMSO is used to disrupt secondary structure formation in the DNA template. This is useful in templates with high GC content.
Aside from its use to aid denaturation of high GC content DNA, DMSO may also be used to loosen up plasmids with supercoiled conformation which also aids in denaturation. It is also advisable to lower the annealing temperature of your PCR reactions when you use DMSO as an additive as it is known to lower primers' melting points. If DMSO doesn't work well to serve its purpose, especially in DNA with very high GC content, other additives such as formamide and glycerol may be used.
Article Dimethyl Sulfoxide-Mediated Primer Tm Reduction: A Method fo...
Hello
Dimethyl sulfoxide (DMSO) is commonly used in PCR to relieve secondary structures when amplifying high GC templates. the addition of DMSO can greatly improve yields and specificities of PCR priming reactions . I do not know exactly what is your purpose with DMSO. If it is investigating the Effects of DMSO on PCR Fidelity, so you will find 5% of DMSO is effective.
Thanks
you can use DMSO to enhance PCR reaction through eliminate secondary structure of DNA and resolve high content of GC in amplicon also you can use other materials like Betaine, DMSO used with 5% of total reaction and you can use 1M of Betaine
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