What is the composition of Wipe buffer?
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05 June 2019 2,941 4 View
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent. During subculturing, to detach the cell from the flask/dish and to avoid cell to cell adhesion...
08 September 2018 3,879 4 View
As I know from researchgate question & answer session; "Split ratio depends on the volume of trypsin + medium to deactivate trypsin + cell" In this regards, my question is this statement correct?...
06 July 2018 1,847 0 View
I am presently planning to do western blot. In addition, I am working H9C2 cell lines. So, I need to prepare sample from cell line. Before to start, I have to prepare lysis buffer. In this...
05 June 2018 5,954 5 View
I am currently working with H9C2 cell lines. I prepared the complete growth media by using following: 1. DMEM 2. 10% FBS and 3. 1% PS Finally, I know media (DMEM) by itself is generally stable for...
05 June 2018 10,067 4 View
In lysis buffer, Sodium fluoride (NaF) act as a serine/threonine phosphatases inhibitor. I found different concentration as well as different storage condition depending on its application over...
05 June 2018 2,919 4 View
I need a complete protocol to prepare fixer solution for X-ray film development. Note: For western blot Enhanced chemiluminescence (ECL) detection method.
04 May 2018 9,976 0 View
I don't know whether this cell line H9C2 is suitable to do the next experient, because i don't know whether H9C2 cell is fully differentiated, could any expertise help me? any test methods?
31 December 2017 5,793 5 View
I am presently working with H9C2 cell. Now, I want to quantify superoxide dismutase enzyme after exposure of hypoxic condition to H9C2 cells. So, which methods should I have to use?
11 December 2017 8,913 1 View
In particularly, I want to know what criteria should I have to consider before selection of a apoptosis methodology? In addition, I am presently working on Ischemic reperfusion induced apoptosis...
11 December 2017 6,376 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?
28 July 2024 5,331 1 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
