01 January 1970 0 726 Report

Focused on your formative years, How to describe your life philosophy?

More Dewan Sumsuzzman's questions See All
How do you find out the most cited paper from Web of Science?

Finding most citated papers in a given topics by using Web of Science tool

05 June 2019 2,941 4 View

How do I prepared 0.25% (w/v) Trypsin­ 0.53 mM EDTA solution?

To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent. During subculturing, to detach the cell from the flask/dish and to avoid cell to cell adhesion...

08 September 2018 3,879 4 View

How much medium is required to deactivate 1 mL trypsin?

As I know from researchgate question & answer session; "Split ratio depends on the volume of trypsin + medium to deactivate trypsin + cell" In this regards, my question is this statement correct?...

06 July 2018 1,847 0 View

Either anionic or non-ionic detergent is suitable for preparing lysis buffer?

I am presently planning to do western blot. In addition, I am working H9C2 cell lines. So, I need to prepare sample from cell line. Before to start, I have to prepare lysis buffer. In this...

05 June 2018 5,954 5 View

What are the shelf-life and optimal storage conditions for cell culture media?

I am currently working with H9C2 cell lines. I prepared the complete growth media by using following: 1. DMEM 2. 10% FBS and 3. 1% PS Finally, I know media (DMEM) by itself is generally stable for...

05 June 2018 10,066 4 View

What is the optimal storage condition of Sodium fluoride (NaF)?

In lysis buffer, Sodium fluoride (NaF) act as a serine/threonine phosphatases inhibitor. I found different concentration as well as different storage condition depending on its application over...

05 June 2018 2,919 4 View

How to prepare the Fixer solution?

I need a complete protocol to prepare fixer solution for X-ray film development. Note: For western blot Enhanced chemiluminescence (ECL) detection method.

04 May 2018 9,976 0 View

How to know the cell H9C2 is fully differentiated?

I don't know whether this cell line H9C2 is suitable to do the next experient,  because i don't know whether H9C2 cell is fully differentiated, could any expertise help me?  any test methods?

31 December 2017 5,793 5 View

How can I choose best methodology to determine apoptosis?

In particularly, I want to know what criteria should I have to consider before selection of a apoptosis methodology? In addition, I am presently working on Ischemic reperfusion induced apoptosis...

11 December 2017 6,376 2 View

Best method for superoxide dismutase measurement ?

I am presently working with H9C2 cell. Now, I want to quantify superoxide dismutase enzyme after exposure of hypoxic condition to H9C2 cells. So, which methods should I have to use?

11 December 2017 8,913 1 View

Similar questions and discussions
How to cacualte the creep life of polycrystal with CPFEM?

I saw a paper(PHAN V-T, ZHANG X, LI Y, et al. Microscale modeling of creep deformation and rupture in Nickel-based superalloy IN 617 at high temperature [J]. Mechanics of Materials, 2017, 114:...

16 July 2024 8,436 0 View

Franklin M. Fisher (1983) Disequilibrium Foundations of Equilibrium Economics. How is it estimated now? How is it related SMD results?

Fisher studied if there is an out-of-equilibrium process that rapidly converges to some equilibrium points. He claims that Hahn process has a Lyapounof function and therefore convergent to an...

15 July 2024 1,460 4 View

Assuming that the central philosophy of the Schrodnger equation is the cat in a box paradox, can this phylosipy be adjusted?

We assume that the cat in a box paradox may allow an additional approach to include multiple cats in the same box. We can now see two seemingly different views, quantum and classical. The...

13 July 2024 8,233 4 View

What are research philosophy and paradigm and importance of quantitative research in philosophy?

What are research philosophy and paradigm and importance of quantitative research in philosophy?

10 July 2024 585 6 View

My new vision on “time duality” in heat and wave equations. How can we catch their solutions and qualitative properties?

Greetings mathematicians and physicists, As we know, the idea of ​​assuming a higher space dimension than the natural 3 dimension is effective in solving many physical and mathematical...

29 June 2024 9,220 6 View

How can I reassgn my expertise in ResearchGate?

I inadvertently 'accepted' a specialisation in biology, but it was the wrong Richard McCullagh. I'm in Australia and he is in the USA. Can I delete that reference and enter 'philosophy of...

23 June 2024 3,124 0 View

Research paradigm of systematic review?

Hi, I am doing a systematic literature review for my MPhil. Does anyone know what philosophy/research paradigm is a systematic review?

18 June 2024 9,525 1 View

Which LCA software would you recommend for comparative LCA of electrolysers? And is ecoinvent database alone is enough for such a assessment?

For additional information, I am a master's student looking for a LCA software to do my thesis. So a less expensive one is my first priority, but if it is not able to help me with a clarity...

18 June 2024 8,376 3 View

My conditional effects are significant but my moderation analysis is not, how come?

So I am doing my thesis on the relation between cyberbullying perpetration and life satisfaction and if physical activity is a moderator. But in my moderation analysis the interaction is...

17 June 2024 6,400 2 View

Welcome Find these basic axes in p df form:1.Introduction to Botany:2.Plant Structure and Function?

conservation Conclusion: In summary, "Exploring Botany: An Inclusive Guide to Plant Biology" aims to be an invaluable resource for students, educators, and enthusiasts interested in delving into...

13 June 2024 5,807 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close