30 Questions 45 Answers 0 Followers
Questions related from Dewan Md. Sumsuzzman
What are the potential reasons that most studies fall outside the 95% confidence interval?
09 July 2024 4,905 2 View
I am creating a forest plot for a meta-analysis and require assistance in adding a superscript after the Author/Year on the left side of the graph. Any help would be greatly appreciated.
10 June 2024 5,254 4 View
I am actively searching one tool/methodology that can assess the quality of published healthcare frameworks, in particularly focusing on existing digital health competency frameworks. Any...
22 October 2022 4,360 0 View
I wonder if anyone provide me a complete list of upper limb and lower limb anti-gravity muscles.
30 April 2019 4,718 11 View
I need a list of neural vs non-neural transcription factors. Have any non-neural transcription factor which has daylight rhythm? Thank in advance who are kind enough to provide me a list of...
30 October 2018 4,716 0 View
I am having trouble, during development of my western blot membranes (PVDF) using the film system, the films become extermly dark when I put them in the developing solution. Brifly I summarize...
19 October 2018 3,731 2 View
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent. During subculturing, to detach the cell from the flask/dish and to avoid cell to cell adhesion...
10 September 2018 9,063 3 View
In lysis buffer, Sodium fluoride (NaF) act as a serine/threonine phosphatases inhibitor. I found different concentration as well as different storage condition depending on its application over...
25 June 2018 7,260 3 View
I don't know whether this cell line H9C2 is suitable to do the next experient, because i don't know whether H9C2 cell is fully differentiated, could any expertise help me? any test methods?
04 January 2018 972 5 View
During Cryopreservation, Why DMSO is not suitable for all cell types? And, therefore why glycerol can be used as an alternative?
22 December 2017 1,732 8 View
According to the abcam protocol, during, measurement of caspase-3 activity we need reaction buffer. So, I want to know what it is composition and basic mechanism of Caspase 3 Assay?
19 December 2017 390 1 View
Uncoupling proteins (UCPs) belong to the superfamily of mitochondrial-inner-membrane anion-carrier proteins. Uncoupling proteins play a role in normal physiology such as it generate heat.
17 November 2017 541 10 View
Why DMSO(Dimethyl sulfoxide) is used in (PCR) polymerase chain reaction?
26 October 2017 6,784 3 View
After SCI, glial scarring by astrocytes inhibit axon regeneration. So, which intracellular components induce glial scar?
26 October 2017 1,579 2 View
Because of gliosis injured axons unable to regenerate which has become a crucial target for regeneration research in Spinal Cord Injury O(SCI). So, I want to know research specific mechanism. If,...
23 October 2017 4,174 2 View
What is the corelation between Irisin & dementia?
10 October 2017 2,686 0 View
Application of stem cell on Spinal Cord Injury animal model
28 September 2017 3,607 2 View
How Spinal Cord Injury affect the Bladder?
28 September 2017 4,796 4 View
According to their application, how can I differentiate between TBS & PBS? As a blocking buffer which one is the best? When? Why?
04 September 2017 9,595 4 View
The datasheet gives us valuable instruction which is very important to complete our experiment successfully. But, sometimes datasheet does not provide everything, for example, primary antibody...
04 September 2017 729 3 View
If gel percentage 15, 12, 10, 7.5, 5 (%) respectively, so what will these gel percentages optimum voltage and time?
31 August 2017 6,737 4 View
Generally, 4°C overnight will give more efficient and specific staining. However, many antibodies will also work very well with one or two hours incubation at room temperature. Actually, I need a...
30 August 2017 1,746 5 View
I know the particular application of BSA & Milk. But, my query, why many laboratories follow the combination of BSA+Milk as a blocking reagent? And, how they get advantages from this combination?
30 August 2017 9,716 3 View
A number of methods had been developed for estimating protein concentration. Which method is most suitable in this case, and why?
30 August 2017 5,030 5 View
Nissl bodies are rough endoplasmic reticulum where proteins are synthesized. They are widely used to reflect the neural structure of the brain and spinal cord and are considered to be related to...
17 August 2017 5,722 0 View
Irisin is a newly discovered myokine which secreted by the muscle contraction during aerobic exercise. Previously a scientific research proved that Irisin can increase intracellular Calcium...
26 July 2017 5,723 3 View
Proinflammatory cytokines are the major culprit to initiate immune response resulting in inflammation. And, exercise mediated myokines have special characteristics against pro-inflammatory...
18 July 2017 3,218 1 View
Definition? How to characterize them? Which software (free) is the best to analyze these outcome measures? How to combine Dichotomous outcome Continuous outcome (If data for the same outcome are...
01 January 1970 9,577 3 View
Does SCI-induced central NP or peripheral NP or both?
01 January 1970 2,904 3 View
Which one is the most prevalent in Alzheimer's disease? How gender difference changes day by day in developed countries? Is one factor impact other?
01 January 1970 344 6 View