According to the abcam protocol, during, measurement of caspase-3 activity we need reaction buffer. So, I want to know what it is composition and basic mechanism of Caspase 3 Assay?
Try this link. It explains the mechanism and the composition of buffers.
https://pdfs.semanticscholar.org/3ef6/8a6944653be3b018bd50224434300951d57b.pdf
Hi! How to handle missing data using multiple imputation in STATA?
03 August 2020 5,530 4 View
Hi! There. How To Drive a Culture of Experimentation For Effective AI (Artificial Intelligence)/ML (Machine Learning)?
02 August 2020 474 4 View
Hi! there. What are the limitations of maximum likelihood estimation procedures when a majority of the observations are below the limit of detection?
02 August 2020 9,391 4 View
Handling imbalanced datasets in machine learning is a difficult challenge, and can include topics such as payment fraud, diagnosing cancer or disease, and even cybersecurity attacks. How to...
28 July 2020 412 14 View
Hi!there. How to perform Stepwise Regression in R or STATA?
20 July 2020 1,093 8 View
Hi there. I have a machine learning project with python by using a scikit-learn library. I have two separate datasets for training and testing and I try to do linear regression. How can I...
20 July 2020 6,131 18 View
What are the differences between data profiling and data mining?
20 July 2020 1,477 16 View
What are the challenges in bridging data science, machine learning, and Accounting and Auditing?
14 July 2020 9,721 14 View
Currently, I am working on coding the Intellectual capital information disclosed in the annual report of the organizations. There are many variables in the literature of intellectual capital and...
25 May 2020 1,855 10 View
MODIS has surface reflectance product too from where we can produce land cover output after supervised classification. From my knowledge of concern Landsat, Sentinel etc. only provide surface...
10 March 2020 8,283 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View