Why I am getting same ct values for three different dilutions?

I am setting up qPCR with human DNA and actin primers, it is working with 10 and 1ng dilution but when I am considering 0.1, 0.01 and 0.001 I a getting same ct values for these 3 dilutions, I am...

08 September 2015 9,674 5 View

Hi, how to get tight pellect from a bacterium which is very slimy?

I am working with a bacterium which is very slimy so I am not able to get the tight pellet, but i need to get tight pellet with very less water for further analysis(pigment analysis)...even i...

01 February 2015 9,703 12 View

This question is deleted by the person who posted it.?

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11 December 2014 6,733 0 View

In gene prediction algorithms are we taking count of enhancers?

I feel the information about enhancers helps us to predict the gene properly, if a gene function is influenced by enhancers then why we r not counting enhancers while predicting gene? some one...

09 October 2014 3,417 6 View

Can any one help me, how do you sequence a virus which contains many modified bases?

Usually bacteriophage contains modified bases, but we don't know what are those modified bases. In above situation how can i go about sequencing such virus.

07 August 2014 8,018 8 View

Can any one please send me original papers on Genetically modified products.Thank you.

I need original papers on Genetically modified products(successful or failed) example:Crops, Food products ,medicinal products. Thank you.

07 August 2014 365 12 View

Hi everyone does anyone have protocols for the following tests?

Acid produced from: Glucose,D-Mannitol,Salicin,Inositol,Sorbitol,Rhamnose,Maltose,D-XYIOX,Trehalose,Cellobiose,Methyl D-glucoside,Erythritol,Melibiose,D-Mannose. Hydrolysis of: Aesculin,...

06 July 2014 6,011 10 View

Is there are any other modified bases in case of T-even Bacteriophages other than Methylation and glucosylation?

Biological DNA modifications have been studied for many years, and much is known about their distribution and the enzymes involved. Well-known base modifications are C-5-methylcytosine (m5C),...

06 July 2014 6,868 1 View

Is it always necessary to memorise everything?

People will always expect that if a person is doing some research they should remember almost all, is it always necessary?

05 June 2014 8,228 2 View

Does anyone have any idea about PFGE (Pulsed field Gel Electrophoresis)?

I do not understand what is happening with the electricity supply (bidirectional), even though the bidirectional bands are very good.. If the electricity supply is bidirectional, then should the...

03 April 2014 1,246 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

The procedure compute?

02 March 2021 5,204 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

If you haven't computer? how you show the photons to pupils?

Educational Process

01 March 2021 1,827 1 View

Please can i get topic and materials that will relate computer with telecommunication companies?

I am a master student and i am working on my research, so I am a bit confuse don't really know how to match computer with telecommunication as a topic

28 February 2021 7,039 6 View