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Questions related from Bhagya Deepachandi
Usually I am doing PCR with 0.5 ul of Taq polymerase (abm) per reaction. Since I wanted to increase the sensitivity of target amplification, I tried PCR with 1.0 ul of Taq. It gave some bands in...
03 March 2018 9,161 6 View
I am doing immunoprecipitation for concentrating by target proteins. I usually elute my proteins in SDS loading buffer containing Tris, glycerol, SDS, beta mercaptoethanol and bromophenol blue....
12 December 2017 1,209 2 View
I have found two commercial ELISA kits for detection of human leishmaniasis. 1. Human Anti-Leishmania IgG ELISA Kit (ab108749) by Abcam 2. Leishmania IgG ELISA Kit (KA3295) by Abnova Both are...
04 April 2017 3,665 1 View
I am facing the a problem in my SDS page gel. protein marker and samples not get well separated. All samples were stop separating at the middle of separating gel (protein marker also). Dye front...
10 October 2016 3,369 12 View
Is there a simple method to physically characterize the crude Ag extracted from a parasite to check whether they are proteins, polysaccharides or phospholipids like compounds? I am using crude Ag...
08 August 2016 3,999 3 View
Does 3D structure of a protein affect for migrating distance of a protein?
07 July 2016 6,277 0 View
Can I keep my blocking buffer (skimmed milk or BSA) at room temperature for overnight? Is that affect for my protein?
06 June 2016 3,938 5 View
I want to sequence some unknown protein excised from 1D-SDS-gel. Have anybody experienced with protein sequencing? Are there any good commercial protein sequencing services?
05 May 2016 4,530 1 View
What are the reasons for getting fuzzy bands on SDS-polyacrylamide gel?
04 April 2016 547 9 View
How can we convert TMB of ELISA for western blot by converting soluble TMB product of ELISA into insoluble with addition of 0.05% sodium nitroprusside? Can I use ELISA TMB substrate for Western blot?
03 March 2016 8,637 0 View
The DAB- white powder, I store at room temperature, desiccator is getting brown colour with the time. It is also not working properly as before (when I have white powder). Is it useless after...
02 February 2016 8,390 2 View
Can anyone suggest a methodology for investigating the vertical resistance transfer mechanisms of insects using insects grown within laboratories?
09 September 2015 9,978 1 View
Can we use HI-FBS as blocking buffer in ELISA?
08 August 2015 5,885 3 View
When we are developing a diagnostic assay, we have to consider about sensitivity and specificity. Is there any standard value for minimum sensitivity of a validated assay?
05 May 2015 7,664 4 View
I have done the first step of optimization of indirect ELISA. It is "optimization of antigen coating". Coating and other procedures are correct. The problem is with evaluating the absorbance...
04 April 2015 7,095 7 View
How we compute normalize absorbance ratio in each ELISA plate?
04 April 2015 9,130 3 View
Are there any different methods to check immuno-dominant activity of an antigen except for ELISA and western blot?
01 January 2015 4,683 2 View
I want to sequence an unknown protein. From where I can get this facility?
01 January 2015 8,584 0 View
Also how do you adjust the volume of each reagent?
01 January 2015 7,837 1 View
I'm using indirect ELISA. To detect IgG antibodies against a specific pathogen. Antigen is also coated by us. The conjugate is an goat anti-human IgG (gamma) antibody. In the assay, healthy...
12 December 2014 2,347 19 View
I used positive and and negative serum for optimizing an ELISA, but there is a difference between each sample. The total antibody content is different from person to person. So, it is not...
12 December 2014 7,532 13 View
I want to do calculations with SPSS for developing diagnostic assays. Are there any reference to study online?
12 December 2014 8,157 3 View
I want to try out the Lowry's method to quantify proteins. When we measure the absorbance, which range of absorbance give most accurate results?
10 October 2014 4,237 3 View
Is it TMB or ABTS?
10 October 2014 6,679 12 View
I want to analyze immuno-dominant antigens of parasites. I extract crude protein and want to check it's immunogenicity using an immunoassay. And I want to find the size of each antigen? is there...
10 October 2014 9,407 3 View
Immunoblot assay make the proteins be denatured? Is that suit for every antigen-antibody interactions?
09 September 2014 9,132 4 View
Does anybody familiarize with the cultivation of leishmania parasites for protein purifications? What is the best culturing condition to obtain optimum growth?
09 September 2014 3,353 9 View
Can I use agarose gel electrophoresis method for proteins separation? How? Which buffers should I use?
08 August 2014 3,951 5 View
I want to store a serum sample (contains antibodies) in lyophilized form for long term. What should be the ideal temperature? Will it affect for destruction of antibodies?
08 August 2014 8,727 1 View
