Can anyone suggest a methodology for investigating the vertical resistance transfer mechanisms of insects using insects grown within laboratories?
It is gene related, use homozygous mutant and wild type insects to check the resistant mechanism. Lot of mechanisms involved in resistance including RNAi. Use hypothesis to check mechanisms via inhibiting pathway enzymes.
Usually I am doing PCR with 0.5 ul of Taq polymerase (abm) per reaction. Since I wanted to increase the sensitivity of target amplification, I tried PCR with 1.0 ul of Taq. It gave some bands in...
02 March 2018 9,088 6 View
I am doing immunoprecipitation for concentrating by target proteins. I usually elute my proteins in SDS loading buffer containing Tris, glycerol, SDS, beta mercaptoethanol and bromophenol blue....
11 December 2017 1,141 2 View
I have found two commercial ELISA kits for detection of human leishmaniasis. 1. Human Anti-Leishmania IgG ELISA Kit (ab108749) by Abcam 2. Leishmania IgG ELISA Kit (KA3295) by Abnova Both are...
03 April 2017 3,589 1 View
I am facing the a problem in my SDS page gel. protein marker and samples not get well separated. All samples were stop separating at the middle of separating gel (protein marker also). Dye front...
09 October 2016 3,280 12 View
Is there a simple method to physically characterize the crude Ag extracted from a parasite to check whether they are proteins, polysaccharides or phospholipids like compounds? I am using crude Ag...
07 August 2016 3,925 3 View
Does 3D structure of a protein affect for migrating distance of a protein?
06 July 2016 6,207 0 View
Can I keep my blocking buffer (skimmed milk or BSA) at room temperature for overnight? Is that affect for my protein?
05 June 2016 3,857 5 View
I want to sequence some unknown protein excised from 1D-SDS-gel. Have anybody experienced with protein sequencing? Are there any good commercial protein sequencing services?
04 May 2016 4,446 1 View
What are the reasons for getting fuzzy bands on SDS-polyacrylamide gel?
03 April 2016 463 9 View
How can we convert TMB of ELISA for western blot by converting soluble TMB product of ELISA into insoluble with addition of 0.05% sodium nitroprusside? Can I use ELISA TMB substrate for Western blot?
02 March 2016 8,565 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...
03 March 2021 6,223 5 View
Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...
02 March 2021 4,910 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...
24 February 2021 8,856 3 View
Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!
24 February 2021 6,805 3 View
This insect is minute in size around 1 mm and not clearly visible with naked eye.
22 February 2021 5,907 11 View
I have a massive of x, y, z coordinates of 2D surface in 3D space, which builded in Ansys HFSS or AutoCAD. How can I get the analitical aproximation of this surface by formulas?
21 February 2021 3,444 7 View
The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...
16 February 2021 1,762 8 View
I want to run 5 different models to estimate stream flow. In order to optimize the characteristics of these models I use Taguchi method. So I have to run different models according to the Taguchi...
11 February 2021 7,986 6 View