Can anyone suggest a methodology for investigating the vertical resistance transfer mechanisms of insects using insects grown within laboratories?
It is gene related, use homozygous mutant and wild type insects to check the resistant mechanism. Lot of mechanisms involved in resistance including RNAi. Use hypothesis to check mechanisms via inhibiting pathway enzymes.
Usually I am doing PCR with 0.5 ul of Taq polymerase (abm) per reaction. Since I wanted to increase the sensitivity of target amplification, I tried PCR with 1.0 ul of Taq. It gave some bands in...
02 March 2018 9,197 6 View
I am doing immunoprecipitation for concentrating by target proteins. I usually elute my proteins in SDS loading buffer containing Tris, glycerol, SDS, beta mercaptoethanol and bromophenol blue....
11 December 2017 1,246 2 View
I have found two commercial ELISA kits for detection of human leishmaniasis. 1. Human Anti-Leishmania IgG ELISA Kit (ab108749) by Abcam 2. Leishmania IgG ELISA Kit (KA3295) by Abnova Both are...
03 April 2017 3,701 1 View
I am facing the a problem in my SDS page gel. protein marker and samples not get well separated. All samples were stop separating at the middle of separating gel (protein marker also). Dye front...
09 October 2016 3,411 12 View
Is there a simple method to physically characterize the crude Ag extracted from a parasite to check whether they are proteins, polysaccharides or phospholipids like compounds? I am using crude Ag...
07 August 2016 4,034 3 View
Does 3D structure of a protein affect for migrating distance of a protein?
06 July 2016 6,313 0 View
Can I keep my blocking buffer (skimmed milk or BSA) at room temperature for overnight? Is that affect for my protein?
05 June 2016 3,976 5 View
I want to sequence some unknown protein excised from 1D-SDS-gel. Have anybody experienced with protein sequencing? Are there any good commercial protein sequencing services?
04 May 2016 4,567 1 View
What are the reasons for getting fuzzy bands on SDS-polyacrylamide gel?
03 April 2016 594 9 View
How can we convert TMB of ELISA for western blot by converting soluble TMB product of ELISA into insoluble with addition of 0.05% sodium nitroprusside? Can I use ELISA TMB substrate for Western blot?
02 March 2016 8,684 0 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...
31 July 2024 3,333 4 View
The aim of the research here is to prevent the propagation of the crack in the fabricated elastic medium with useful applications.
25 July 2024 9,976 3 View
In recent years, quantum computing has emerged as a groundbreaking technology with the potential to revolutionize various fields, including artificial intelligence (AI). AI has already made...
17 July 2024 1,398 3 View
Hi All I recently get admission in PhD statistics. After a long discussion with my supervisor, the topic I selected for PhD is " Air Pollution and its impact on Economy: A case study of...
15 July 2024 1,820 5 View
Is there a way to add new Journals in the system?
03 July 2024 1,691 2 View
Can anyone know how to overlay 3 or more crystal structure/chamical structures by using any software like olex2, mercury or pymol. Mercury has a option to do.. i want to know the step by step process.
16 June 2024 6,871 0 View
I'm a student of biological sciences and I need to collect information about how ambient temperature affects insects behaviour to answer the question: " which insects are more active in...
15 June 2024 367 3 View