My primer has 95.05 ug, 3.25 OD, 6124.99 MWT and 15.51 nmol
The initial conc we need 10 uM
The final conc 5 uM
what I understand from online that I will prepare a stock soln first by adding 10 times distilled water
So 15.51 + 155.1 dW
Then prepare the working soln by adding 10 ul of stock with 90 ul of water.
Is this method right?
I started to answer you question a few time but it is a little confusing. The second part is easier to understandif you add 10% of stock with 90 % water you will reduce almost always the concentration be a factor of 10. It is a little more proper to start with the 10 ul of staok and add water and add water until you have a total of 100 uL because some there are some mixtures that expand or contract a little when you mix them up.
Now the confusing part in not sure what " My primer has 95.05 ug, 3.25 OD, 6124.99 MWT and 15.51 nmol means and why are you diluting with 10 times as much dW instead of 9? I wil wait for clarification before I try to answer that part of the question.
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