Hello every body,
Id like to inject mouse recombinant sonic hedgehog subcutaneously in mouse and the dose will be 1 mg / kg (0.02 mg for 20 gm mice)my question is What is the best way to inject this little dose
Also if any one has experience can i make a dilution to it before injection without change the efficacy of the dose
Shveta Sharma
In my opinion dilution is not good option. Try to injection dose by syringe and needle used for giving insulin injection or if you are having facilities give dose by infusion pump which is mostly used for i v infusion but can also be used for s c injection.
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