How can we evaluate physically the effect of Sympathetic denervation in mice?

More Ayman Khalifa's questions See All

Good evening everyone can anyone send me SMC files of earthquakes of different intensities for a simulation on Plaxis 2D?

Good evening everyone can anyone send me SMC files of earthquakes of different intensities for a simulation on Plaxis 2D. Thanks

02 March 2024 4,362 1 View

Was the name of a researcher from Helwan University published on the website of most of my articles?

أخاطب السيدة المذكورة باسم Sarah Ayman بحذف اسمها من على الأبحاث الخاصة بى لأن هذا يعد انتحال علمى وسوف نبلغ جامعة حلوان بذلك

25 February 2024 7,614 1 View

We ask you to kindly change the information of the first name written incorrectly?

We ask you to kindly change the information of the first name written incorrectly as Sarah Ayman to the correct name as listed in the article, Ayman S Taha. Sincere thanks and appreciation.

25 February 2024 8,634 1 View

In the flotation process, what is the future effectiveness of using an activator reagents on copper oxide surface?

I know that many reagents are used as activators or enhancers (such as ammonium salts and so on) in copper oxide minerals processing to facilitate their flotability during the flotation process....

28 December 2023 2,694 0 View

In what cases is the phosphate group attached to carbon atom number 2 in nucleic acids?

I found this paragraph on page 223, Chapter 10, Concepts of Genetics. "The phosphate group may be bonded to the C@2′, C@3′, or C@5′ atom of the sugar"

27 September 2023 7,074 0 View

Transfection of neuroblastoma cell line (SHSY5Y) for RNA collection??

Usually neuroblastoma have low transfection efficacy (5-10%) when transfected with Lipofectamine 3000. So, this time I have tried electroporation with this condition, poring pulse: 175V, 2.5ms,...

23 August 2023 1,962 2 View

How to calculate the optimum annealing temperature for PCR?

15 August 2023 2,205 5 View

Since carbohydrates and lipids are macromolecules, why did no one think in the past that one of them was the genetic material?

Why was it believed in the past that the genetic material was either DNA or protein? why did no one think that carbohydrates or lipids were the genetic material? Why all the research and theories...

18 July 2023 7,177 1 View

Why do supercapacitors sometimes struggle to reach the voltage cutoff at low current densities?

Any solid reference to why sometimes, especially at very low current densities, it takes too long to reach the charging voltage cutoff, while discharge takes place much faster?

03 July 2023 5,583 3 View

Any one interesting to join our Food Molecules Interaction BOOK??

Dr. Khalifa, Prof. Zou, and Prof. Soottawat in collaboration with Elsevier Publisher warmly welcomed all professional scientists to join us in this book proposal to write and/or suggest one...

21 May 2023 2,978 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Subhash C. Juneja

I am enclosing one basic paper. That may set you thinking on characterization of denervation. There will be many such papers, you can find out from Pubmed and get the right one.

USC has good neuro program, check with some PI working in this area.

Best wishes,

Ayman Khalifa

thanks for your paper

its really helpful

appreciated

Alessio Rotini

Hello, if you want some tips I can help you. I work with this drug. All the best, Alessio