Does anyone worked with CGRP on mice. I m stuck with which the product good to use.
I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat# of the product they used in their studies.
Hope I could get a satisfied guide to my question
Thank you
Dear Ayman!
Please You look at following articles:
https://www.jbc.org/article/S0021-9258(19)36796-1/pdf
Cell Clones and Lines-The murine clones 5.9 and 5.5 are TH1 cells
The murine TH~ cell Dl0 G4.1 is a T cell clone w
Cells 5.5 and Dl0 G4.1 were also cultured at 5 X lo6 cells/ml for 24 h in the presence of ConA and conalbumin (2.5 pg/ml), respectively, and tested for their proliferative response to CGRP and for their IL-4 and IL-2 production.
https://link.springer.com/content/pdf/10.1186/s12953-018-0146-4.pdf
Cell treatments The experiments were divided into two parts. The first part was designed to develop an initial understanding of the effects of CGRP (alpha type, Abcam, ab47101) on EPC proliferation and apoptosis. An equal volume of PBS was added to the control group, and 10− 8 M, 10− 10 M, and 10− 12 M CGRP (final concentration) was added to the experimental groups [22, 23, 33].
