Can anyone tell me the relationship of gene expression in base pairs and amplicon size?

I wish to know is it possible to correlate the expression of a gene in base pairs with its amplicon size.

09 October 2017 6,242 0 View

Can anyone suggest me a program which can be used to check whether a particular gene sequence may be working or not?

10 November 2016 5,294 7 View

Did anyone know whether Heart type fatty acid binding proteins and Liver fatty acid binding proteins are present in HL-60 cells?

I am working on some fatty acids. I wish to know whether FABPs of Heart and Liver types are present in HL-60 cells.

08 September 2016 9,216 1 View

Can anyone suggest me an useful software that can be used to check whether a given DNA sequence is working or not?

As part of my research work, I have to check the expression of some genes. However when I go through references, I got different sequences for the same gene. I wish to know which of these...

08 September 2016 5,137 4 View

Can Propidium Iodide uptake assay be done in rat heart/ liver tissues?

As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.

07 August 2016 6,155 3 View

Can anyone suggest me some good kits for performing TUNEL assay in vivo?

As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?

07 August 2016 2,971 2 View

Can anyone provide me the calculation for SOD using the Method of Kakkar et al., (1984)?

I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...

06 July 2016 3,290 1 View

Can anyone please suggest to me a method for isolating cell membranes?

I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...

02 March 2016 6,495 1 View

Is there any simple method which serves as a marker assay for inflammation instead of COX-2 and LOX-5?

Hello, I am working on cell culture. I wish to know if there is any simple method which serve as marker of inflammation instead of Cyclooxygenases and lipooxygenases.

11 December 2015 6,901 4 View

Is it suitable to carry out centrifugation in normal centrifuges (instead of cooling centrifuge) while doing enzymatic assays?

Hello, I wish to know Whether it is good to carry out centrifugation in normal centrifuges instead of cooling centrifuges while doing enzymatic assays especially in cell culture.

10 November 2015 8,195 4 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View