Abdul Rehman

15 Questions 15 Answers 0 Followers

Questions related from Abdul Rehman

Sodium dodecyl sulfate 0.1% can be 100% removed from solution by centrifugation?

I have received a nanoparticle suspension from a manufacturer, which contained SDS 0.1% in stock solution. I doubt that it may influence the experiment, therefore i want to remove the SDS from...

25 January 2024 7,669 0 View

In article publication combined results & discussion should be preferred? or separated! what are the scientific&technical reasoning in this aspect?

One of the reviewer asked me to split results and discussion section, since I still want to continue the the article submission with combined results and discussion... please share your knowledge...

22 January 2024 1,940 5 View

How to design graphical abstract and if specific software recommendations?

I would like to articulate graphical abstract for adsorption of pollutants by different absorbents from water and 3D materials.

01 December 2023 5,859 4 View

Why Nanoplastics at low concentration are more toxic than high concentration? What is your opinion..?

Suppose 100 nm NPs at high an low concentrations but nano particle size is same .. how ever impact is always there either its low or high. Lets join interesting open debate and collect the...

16 December 2022 2,263 5 View

Which software can be used to plot PCA, PLS-DA and OPLS-DA data?

Please suggest me appropriate software or other source.

25 April 2022 9,252 6 View

How to prepare/Extract nanoparticles from tissues for MALDI-TOM-MS?

I want to determine nanoparticle accumulation in tissue sample by using MALDI-TOF-MS, but I could not find methods related to to tissue samples. I would like you to share your knowledge or...

11 January 2022 8,463 4 View

Can I store tissue sample for long time, in aqueous methanol:water v/v, after as soon as sample collection for metabolomics using GC/MS?

We do not have GC/MS available in our Lab, therefore I need to send Tissue samples to the company for metabolomic analysis. The out source Lab has to perform derivatization and extraction and...

15 November 2021 5,784 3 View

Is this possible to design forward and reverse primers by using gene gene accession number/code?

I am looking for target gene forward and reverse primers form published literature but it available information is limited because it lacks number of nucleotides/bp and qPCR protocol for each...

02 November 2021 2,292 6 View

Can i obtain differentially expressing genes (DEGs) from Samples extracted through FastDNA spin Kit for Soil?

Initially I extracted DNA by using "FastDNA spin Kit for Soil" and and confirmed DNA concentration in sample by using Nanodrop. The DNA extracted sample were purposed to asses microbial diversity...

20 October 2021 4,074 3 View

How to determine metabolites within animal intestine by using GC/MS?

For example: The intestine/gut of zebrafish as model organism. I would like to study metabolic profiling by using GC/MS. Could you please suggest suitable protocol for determining metabolites in...

13 October 2021 6,023 3 View

I am looking for commercial source can provide us 13C-Labelled with nano or micro plastic materials???

Dear Scholars, please recommend me the source link or contacts . Thanks in advance.

30 June 2021 5,207 1 View

If a nano particle's surface area is 25 m2(square)/g, then can we measure its exact size in nm?

I have nano powder material but i do not know the exact nm size, however it is understood nano powder consist of various different nm size, but if surface area of particle is known then can find...

15 September 2020 5,857 8 View

Can I substitute Glutaraldehyde (GA) with other alternatives such as paraformaldehyde , ethanol to fix sludge sample for SEM?

I would like to fix OR prepare activated sludge samples for scanning electron microscopy (SEM). I would like to replace Glutaraldehyde (GA) but i am unaware of concequences.

17 September 2019 3,554 2 View

What Formamide concentration in the hybridization buffer can be used for probe hybridization in situ?

Because different probes require different formamide concentration in FISH protocol. I would like to know subsequent formamide concentration for the oligonucleotide RNA probes given...

06 June 2019 3,406 1 View

How to inhibit Glycogen-accumulating organisms (GAO) in activated sludge waste water treatment process?

i would like to know if there is recently new technique developed to inhibit the GAO activity without affecting phosphate accumulating organisms (PAO). Please share your knowledge

10 April 2019 5,074 5 View