For example: The intestine/gut of zebrafish as model organism.
I would like to study metabolic profiling by using GC/MS. Could you please suggest suitable protocol for determining metabolites in tissues.
Thanks in advance.
In this work, a proper extraction methodology is described. Please see section 2.6.
Article Identification of multiple dysregulated metabolic pathways b...
Erhan Akkaya Thank you so much sir, for sharing value-able information, it is very helpful.
You need to perform derivatization step with BSTFA (1h 60ºC)
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or we can collaborate
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