Can I substitute Glutaraldehyde (GA) with other alternatives such as paraformaldehyde , ethanol to fix sludge sample for SEM?

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Nicolas Barois

Hello,

Glutaraldehyde is the best but you can use paraformaldehyde.

Avoid ethanol, acetone. They fix by precipitation, so you may have deformation of your soft sample.

Paraformaldehyde is preferably use for immunofluorescence experiments with a short fixation time (15 minutes) to allow immunolabelling.

For SEM you can fix your samples much longer (hours, days).

Abdul Rehman

Nicolas Barois Thank you and appreciated.