I have received a nanoparticle suspension from a manufacturer, which contained SDS 0.1% in stock solution. I doubt that it may influence the experiment, therefore i want to remove the SDS from solution which is originally in ultrapure water?
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
students already took 1. numerical methods 2. programming language 3. Probability and statistics
09 July 2024 6,271 3 View
To generate curve but a straight line generated on zero load.
09 July 2024 2,617 1 View
Hi, my research involves in ZnO synthesis. I have performed rietveld refinement of my samples and obtain a good fit using Fullprof suite software. However to obtain the crystallite size, the IRF...
07 July 2024 3,526 3 View
we need surface area, corrosion current, equivalent weight and density of the metal involved for corrosion rate calculation in mm/year (lets say). For dissimilar metal welds, how can we measure...
30 June 2024 5,943 2 View
Respected researcher, I want to know that is there any way or one can help in generating a 2D/3D image using the energy count spectrum of from backscatter methods. How can I design such an...
29 June 2024 10,023 1 View
Consider the impact of policy stability, corruption levels, and government efficiency on economic performance in both types of governance. Economic growth is a critical indicator of a country's...
21 June 2024 4,786 3 View
I do a research about Non-Verbal Communication: Gestures and Body Language in Classroom.
18 June 2024 8,737 1 View
In my XPS spectral, i detected oxygen group bonded to carbon and in my zinc spectral (note : my sample is ZnO) ...there are two valent zinc species available, (which is unsual as my previous...
17 June 2024 3,888 2 View
Action research design is Quantitative Studies or Qualitative Studies?
14 June 2024 1,684 1 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?
01 August 2024 723 0 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
isolation of microplastic from sludge sample using centrifugation ..
23 July 2024 6,418 0 View