21 Questions 44 Answers 0 Followers
Questions related from Abbas Raza
Hi there Has anyone done a titration curve looking at the amount of MOG peptide used to immunize C57BL/6 for EAE disease. I assume there is development of anergic T cells once a threshold is...
26 September 2017 9,138 1 View
I've some SNP sequence data (15Mb) to analyze and map with segregation of a phenotype in mouse however I am finding it hard to identify haplotype blocks visually from the excel files. Does anyone...
11 July 2016 787 1 View
Hi, i am planning to measure some cytokines in mouse serum at different time points in my model and was wondering if anyone has done these retro-orbital blood collection and the amount that one...
10 September 2015 2,460 2 View
I would like to get measured DHT in mouse serum, we tried one human competition ELISA and it didn't work. Any suggestions for commercial sources to try?
13 August 2015 4,529 3 View
Hi, I am looking for the DHT hormone levels in a B6 mice. I see levels of around 1pg/ml but haven't seen any literature quoting that. Anyone measured it in serum? Thanks.
12 August 2015 324 2 View
Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no...
17 February 2015 7,884 5 View
poly A tails are required partly for mRNA stability. i am wondering if anyone has a clue about why histone mRNA lack such modification?
08 February 2015 285 3 View
I want to know if anyone has made a N-terminal tagged FLAG expression construct of histamine receptor 1. I wish to express the full length protein but wondering if I need an extra linker between...
14 November 2014 3,572 3 View
Has anyone looked at the trafficking of histamine receptor 1 from cytoplasm to cell surface? I am interested to know if there are putative SRP sequences in HRH1. Any thoughts? Abbas
12 November 2014 5,735 0 View
Hi, I am looking for the dosage of this agonist methylhistaprodifen and dimethylhistaprodifen in mice in mg/kg. I haven't yet found any dosage in other species as well. Has anyone worked with this...
20 October 2014 850 1 View
Has anyone used the specific Histamine receptor 1 agonist 2-Pyridylethylamine dihydrochloride in mice and what dosage? I haven't found any literature using this drug in a mouse model. Any help?
01 October 2014 8,875 2 View
I am looking for a good review on secretion system in archaea and how the export machinery differs in terms of SecYEG, SecA, SecB system. Has anyone come across a good review in this area?
28 September 2014 3,569 3 View
I am working on identifying phosphorylation site (s) within a novel protein and and their roles. It appears through mass spectrometer of my protein that there are phosphorylated serines,...
10 July 2014 4,561 4 View
I don't have an idea about mass specs of proteins but am curious to know if it is possible to have a relative abundance of phosphorylated/unphosphorylated protein from mass spec data?
10 July 2014 431 5 View
I am looking for a good explanation on transcription and translation strategy used by NDV. I searched for a good review but couldn't find anything on replication and translation. Has anyone come...
28 June 2014 5,302 2 View
I am looking to work on an experiment and need some information about cell population of mast cells in lymph nodes and spleen. which one has more?
15 May 2014 3,475 3 View
I am having a tough time figuring out problems in my PCR where I am using a set of mouse IL2 primers and amplifying a segment. I am getting amplification with no template as well. I changed all...
08 May 2014 4,842 19 View
I came across a figure and was wondering which cells are sorted by CD4-/CD8- cells.
16 April 2014 2,617 4 View
Commensals provide the tonic signalling for effective innate and adaptive immune responses. After all, except for a few bad guys all are good for us! This is a hot topic and I am looking for...
01 April 2014 7,238 4 View
Either 3'RACE or 5'RACE.
02 March 2014 4,345 9 View
I am interested in the role of invariant Natural Killer T cells (iNKT) in the initial response to HBV infection inside hepatocytes. I have seen this cell line used in many such studies so I...
02 July 2013 1,383 2 View
