Abbas Raza

21 Questions 44 Answers 0 Followers

Questions related from Abbas Raza

MOG35-55 induced EAE: amount of peptide needed?

Hi there Has anyone done a titration curve looking at the amount of MOG peptide used to immunize C57BL/6 for EAE disease. I assume there is development of anergic T cells once a threshold is...

26 September 2017 9,134 1 View

Looking for some guidance on haplotype association mapping?

I've some SNP sequence data (15Mb) to analyze and map with segregation of a phenotype in mouse however I am finding it hard to identify haplotype blocks visually from the excel files. Does anyone...

11 July 2016 783 1 View

How much Retro-orbital blood can we safely withdraw from mice?

Hi, i am planning to measure some cytokines in mouse serum at different time points in my model and was wondering if anyone has done these retro-orbital blood collection and the amount that one...

10 September 2015 2,455 2 View

Can anyone suggest commercial source that does hormone measuements from mouse serum samples in US?

I would like to get measured DHT in mouse serum, we tried one human competition ELISA and it didn't work. Any suggestions for commercial sources to try?

13 August 2015 4,525 3 View

Approximate range of serum DHT (Dihydrotestosterone) in B6 mice?

Hi, I am looking for the DHT hormone levels in a B6 mice. I see levels of around 1pg/ml but haven't seen any literature quoting that. Anyone measured it in serum? Thanks.

12 August 2015 320 2 View

Has anyone come across S-myc tag rather than the conventional c-myc protein tag? Can we detect this protein tag using anti-c-myc antibodies or not?

Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no...

17 February 2015 7,880 5 View

Why do histone mRNAs lack poly A tails?

poly A tails are required partly for mRNA stability. i am wondering if anyone has a clue about why histone mRNA lack such modification?

08 February 2015 280 3 View

Has anyone made a FLAG linker for histamine receptor 1?

I want to know if anyone has made a N-terminal tagged FLAG expression construct of histamine receptor 1. I wish to express the full length protein but wondering if I need an extra linker between...

14 November 2014 3,568 3 View

Is anyone familiar with the trafficking of histamine receptors?

Has anyone looked at the trafficking of histamine receptor 1 from cytoplasm to cell surface? I am interested to know if there are putative SRP sequences in HRH1. Any thoughts? Abbas

12 November 2014 5,730 0 View

What is the In-vivo dosage of H1 receptor agonist methylhistaprodifen and dimethylhistaprodifen?

Hi, I am looking for the dosage of this agonist methylhistaprodifen and dimethylhistaprodifen in mice in mg/kg. I haven't yet found any dosage in other species as well. Has anyone worked with this...

20 October 2014 848 1 View

What is the dosage of H1R agonist 2-Pyridylethylamine dihydrochloride?

Has anyone used the specific Histamine receptor 1 agonist 2-Pyridylethylamine dihydrochloride in mice and what dosage? I haven't found any literature using this drug in a mouse model. Any help?

01 October 2014 8,873 2 View

Is anyone familiar with protein export in archaea?

I am looking for a good review on secretion system in archaea and how the export machinery differs in terms of SecYEG, SecA, SecB system. Has anyone come across a good review in this area?

28 September 2014 3,563 3 View

Phosphoserines vs phosphotyrosines vs phospho threonines...which one can we detect more easily?

I am working on identifying phosphorylation site (s) within a novel protein and and their roles. It appears through mass spectrometer of my protein that there are phosphorylated serines,...

10 July 2014 4,558 4 View

Can anyone help with quantitative mass spectrometry of proteins?

I don't have an idea about mass specs of proteins but am curious to know if it is possible to have a relative abundance of phosphorylated/unphosphorylated protein from mass spec data?

10 July 2014 427 5 View

Where can I find a NewCastle Disease Virus (NDV) replication query?

I am looking for a good explanation on transcription and translation strategy used by NDV. I searched for a good review but couldn't find anything on replication and translation. Has anyone come...

28 June 2014 5,298 2 View

Which has more mast cells? Lymph nodes vs spleen

I am looking to work on an experiment and need some information about cell population of mast cells in lymph nodes and spleen. which one has more?

15 May 2014 3,472 3 View

Can anyone help with an issue regarding PCR? The primers are amplifying in negative control.

I am having a tough time figuring out problems in my PCR where I am using a set of mouse IL2 primers and amplifying a segment. I am getting amplification with no template as well. I changed all...

08 May 2014 4,837 19 View

Which cells are represented by CD4-/CD8- cells ?

I came across a figure and was wondering which cells are sorted by CD4-/CD8- cells.

16 April 2014 2,613 4 View

How beneficial are commensals in maintaining the balance of our immune system?

Commensals provide the tonic signalling for effective innate and adaptive immune responses. After all, except for a few bad guys all are good for us! This is a hot topic and I am looking for...

01 April 2014 7,233 4 View

Can anyone provide a good animated explanation of R.A.C.E--PCR technique?

Either 3'RACE or 5'RACE.

02 March 2014 4,341 9 View

I would like to know about RMAS cell line and how it is defective in iNKT cell activation. Can someone tell me from where can I get these cells?

I am interested in the role of invariant Natural Killer T cells (iNKT) in the initial response to HBV infection inside hepatocytes. I have seen this cell line used in many such studies so I...

02 July 2013 1,379 2 View