27 Questions 51 Answers 0 Followers
Questions related from Abbas Raza
Hi i tried the UCSC lift over tool to find syntenic region been human and mouse genomes using the respective genomic coordinates. That tool however give half the queries as missing synteny. Is...
11 January 2018 2,736 1 View
Hi there Has anyone done a titration curve looking at the amount of MOG peptide used to immunize C57BL/6 for EAE disease. I assume there is development of anergic T cells once a threshold is...
26 September 2017 9,180 1 View
We have a mouse model of anaphylaxis and we are interested to measure cardiac output, aortic blood flow(ABF), PAP, LAP, SAP, central venous pressure. I was wondering if anyone has expertise in...
26 April 2017 6,379 0 View
I've some SNP sequence data (15Mb) to analyze and map with segregation of a phenotype in mouse however I am finding it hard to identify haplotype blocks visually from the excel files. Does anyone...
11 July 2016 836 1 View
Hi, i am planning to measure some cytokines in mouse serum at different time points in my model and was wondering if anyone has done these retro-orbital blood collection and the amount that one...
10 September 2015 2,516 2 View
I would like to get measured DHT in mouse serum, we tried one human competition ELISA and it didn't work. Any suggestions for commercial sources to try?
13 August 2015 4,569 3 View
Hi, I am looking for the DHT hormone levels in a B6 mice. I see levels of around 1pg/ml but haven't seen any literature quoting that. Anyone measured it in serum? Thanks.
12 August 2015 368 2 View
I am looking for a couple of ways an exosome vesicle is able to move across BBB and release its cargo to target cells in CNS.
14 June 2015 243 6 View
Hi there Has anyone looked at the effect of surgical castration (orchiectomy) in male C57BL/6 and how it affects the development of EAE (experimental autoimmune encephalomyelitis)? I've not found...
28 May 2015 179 1 View
Has anyone come across using S-myc tag rather than the conventional c-myc protein tag? I received a construct that had S-myc and I am not sure if this is the same tag as c-myc. There is no...
17 February 2015 7,948 5 View
poly A tails are required partly for mRNA stability. i am wondering if anyone has a clue about why histone mRNA lack such modification?
08 February 2015 342 3 View
I want to know if anyone has made a N-terminal tagged FLAG expression construct of histamine receptor 1. I wish to express the full length protein but wondering if I need an extra linker between...
14 November 2014 3,624 3 View
Has anyone looked at the trafficking of histamine receptor 1 from cytoplasm to cell surface? I am interested to know if there are putative SRP sequences in HRH1. Any thoughts? Abbas
12 November 2014 5,793 0 View
Hi, I am looking for the dosage of this agonist methylhistaprodifen and dimethylhistaprodifen in mice in mg/kg. I haven't yet found any dosage in other species as well. Has anyone worked with this...
20 October 2014 896 1 View
Has anyone used the specific Histamine receptor 1 agonist 2-Pyridylethylamine dihydrochloride in mice and what dosage? I haven't found any literature using this drug in a mouse model. Any help?
01 October 2014 8,921 2 View
I am looking for a good review on secretion system in archaea and how the export machinery differs in terms of SecYEG, SecA, SecB system. Has anyone come across a good review in this area?
28 September 2014 3,619 3 View
I work with 100 microns mouse mesenteric arteries and am trying to mount them on glass cannulas but my technique is not good. I frequently get the artery damaged or break the cannulas. I am...
23 July 2014 4,807 8 View
I am working on identifying phosphorylation site (s) within a novel protein and and their roles. It appears through mass spectrometer of my protein that there are phosphorylated serines,...
10 July 2014 4,614 4 View
I don't have an idea about mass specs of proteins but am curious to know if it is possible to have a relative abundance of phosphorylated/unphosphorylated protein from mass spec data?
10 July 2014 483 5 View
I am looking for a good explanation on transcription and translation strategy used by NDV. I searched for a good review but couldn't find anything on replication and translation. Has anyone come...
28 June 2014 5,361 2 View
I am looking to work on an experiment and need some information about cell population of mast cells in lymph nodes and spleen. which one has more?
15 May 2014 3,529 3 View
I am having a tough time figuring out problems in my PCR where I am using a set of mouse IL2 primers and amplifying a segment. I am getting amplification with no template as well. I changed all...
08 May 2014 4,897 19 View
Can anyone give me a good explanation or annotated figure of where this technique is used to knock in any gene at embryonic stage?
21 April 2014 1,761 5 View
I came across a figure and was wondering which cells are sorted by CD4-/CD8- cells.
16 April 2014 2,672 4 View
Commensals provide the tonic signalling for effective innate and adaptive immune responses. After all, except for a few bad guys all are good for us! This is a hot topic and I am looking for...
01 April 2014 7,282 4 View
Either 3'RACE or 5'RACE.
02 March 2014 4,400 9 View
I am interested in the role of invariant Natural Killer T cells (iNKT) in the initial response to HBV infection inside hepatocytes. I have seen this cell line used in many such studies so I...
02 July 2013 1,437 2 View
