I am looking to work on an experiment and need some information about cell population of mast cells in lymph nodes and spleen. which one has more?
In naive mice - neither LN nor spleen have many mast cells. Nearly undetectable by tissue histology. Here is example for spleen Am J Pathol. Sep 2005; 167(3): 835–848.doi: 10.1016/S0002-9440(10)62055-X
For nodes, I have not done histology but this paper suggests it depends on the node. J Immunol. Apr 1, 2008; 180(7): 4648–4655.
I've seen other papers that use flow mast cells in naive nodes and find < 0.1% of CD45+ cells are mast cells. This is also node site dependent. Skin draining nodes are more mast cell rich than other sites.
As expected, lymph nodes should have more mast cells as noticed by us during examination of spleen and lymph nodes of several animal species. Regional differences in mast cell numbers in lymph nodes do exist and mesenteric as well as bronchial lymph nodes have usually more mast cells, besides sub-cutis nodes. Usually a few mast cells in lymph nodes are visible in young and growing animals rather than adults, wherein the nodes are regressed. Animals suffering from localized or generalized allergic or hypersensitivity reactions or parasitic infections usually show increased mast cell numbers in draining lymph nodes.
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