I came across a figure and was wondering which cells are sorted by CD4-/CD8- cells.
If you are talking about CD3+ CD4- CD8- cells, they are probably immature thymocytes as suggested by Dominic. If your cells are CD45+ but CD3- CD4- and CD8- they are probably innate lymphoid cells (ILCs) also depending on the tissue they come from. Could you give us the right figure?
Naïve H1H2RKO and H3H4RKO mice exhibit normal immune systems. Single-cell suspensions of thymus, lymph node, and spleen were generated from B6, H1H2RKO and H3H4RKO mice. (A) The total number of thymic, lymph node, and spleen cells was enumerated and shown as mean + SEM of n = 10 per strain. Flow cytometric analysis of immune cell subpopulations in (B) thymus, (C) lymph node and (D) spleen of B6, H1H2RKO, and H3H4RKO mice was also performed and shown as mean + SEM of n = 10 per strain. (E) The frequency of Foxp3+ cells in thymus, spleen, and lymph node was also determined and shown as mean + SEM of n = 10 per strain. Significance of differences was determined by ANOVA followed by Bonferroni post-hoc multiple comparison (**p < 0.01; ***p < 0.001). Data are representative of two independent experiments.
Combinatorial roles for histamine H1-H2 and H3-H4 receptors in autoimmune inflammatory disease of the central nervous system
Eur J Immunol. 2012 June;42(6):1536-1546.
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