Article Bacillus subtilis YhaM, a Member of a New Family of 3'-to-5'...
There are other nucleases also known to be Ca or Mn dependent. For example the Staph nuclease (micrococcal nuclease) is Ca2+ dependent. So this isn't a surprise.
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...
30 July 2024 8,339 2 View
Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco
29 July 2024 2,163 1 View
I work on producing organic fertilizer from rice husk. how can I reduce the bio-conversion time ?less than two months
20 July 2024 6,049 3 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
Hello, I'm trying to create parameter and coordinate files for a drug (PRG-A01) found on the following page: http://www.probechem.com/products_PRG-A01.html I used the guide made by Michael...
17 July 2024 3,472 3 View
This is the code I have. I would need: An initial configuration (position and momentum), the orientation of the ecliptic with respect to the direction of travel by the sun, The orientation of the...
09 July 2024 6,063 2 View
eliminoar articulos que son de tu autoria
05 July 2024 2,107 1 View
I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...
01 July 2024 2,124 2 View
Does anyone here know how to export publications (from ResearchGate, ORCID, Google Scholar or EndNote) to a LinkedIn profile? I would like to avoid adding each paper manually. Thanks.
12 May 2024 9,307 4 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,847 1 View
I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...
27 June 2024 2,951 5 View
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15 June 2024 6,072 2 View
This jelly gets precipitated during ammonium sulfate precipitation
06 June 2024 5,631 4 View
Hi, i am doing research on the potential antimicrobial activity of Bacillus against s.aureus.i have tried various methods for six months.. like soft agar overlay method but my bacillus colony keep...
26 May 2024 2,151 6 View
I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...
02 May 2024 4,928 0 View
As a new microbiologist, I am currently working in a non-sterile production pharmaceutical factory where I take pure water samples from 13 points, fill 100 ml of pure water into 3 sample...
29 April 2024 7,096 1 View
I have some environmental isolates that we are looking to identify. Morphologically, the majority of the isolates are Bacillus spp. We would like to use the API 50 CHB/E system and would like to...
28 April 2024 9,559 0 View
Please provide an answer along with the reference.
26 March 2024 7,488 2 View
I and using vectors with autoinducibe promoters(Pylb and cyt1). I have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.
14 March 2024 8,752 0 View