Yeast ribosome preps

More Rasha Al-attar's questions See All

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

Agglomerated Green synthesized CeO2?

Dear colleagues i have synthesized CeO2 using green method and now the Np are not soluble in water how can I make it soluble in water? Best Regards

18 March 2024 4,496 2 View

Biocompatible stabilizers soluble in pbs?

Dear colleagues what are the biocompatible stabilizers that are soluble in PBS and can be used in anti cancer treatments?

15 March 2024 8,003 1 View

Can you help me by answering the following questionnaire?

Hello dear teachers, I am currently conducting a research study for my master's degree, and I need your valuable contributions during this process. My survey is designed to explore factors that...

19 February 2024 1,138 0 View

Zero point charge?

How to determine the zero point charge for a schottky barrier or a composite consisting of more than one oxide for photocatalytic degradation ? Thanks

16 December 2023 478 6 View

How to best analyse a sensory evaluation records?

Hello all, Hope this message finds you in good health. I am trying to analyze sensory data "9-hedonic scale" using RStudio, What is the best analysis to use, and is there a script I can...

18 November 2023 3,002 3 View

How to prevent the aggregation of positively charged nanoparticles in the aqueous environment?

I have made positively charged nanoparticles with Lecithin, Squalene, Tween 80, Span 85 and Dotap. But after using ultrasonic, the particles are aggregated and their size increases. How can...

10 September 2023 3,280 3 View

Hi, what is the problem?

I made cationic nanocarrier by lecithin, Squalen, tween 80, span 85 and DOTAP via melting method in 80 C, but nanoparticles had negative charge instead of positive charge. Does anyone know where...

04 August 2023 5,742 1 View

Can lipid nanoparticles larger than 200 nm pass through the 0.2 filter due to flexibility?

Hi, Can lipid nanoparticles larger than 200 nm pass through the 0.2 filter due to flexibility?

04 August 2023 2,727 2 View

Photothermal therapy off time ?

Dear colleagues…. why we use the laser off time to calculate the photothermal conversion efficiency not the on time? Best Regards

25 June 2023 3,843 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Reshma Alokam

Check out these links

http://www.illumina.com/applications/sequencing/rna/ribosome-profiling.ilmn

http://www.cambio.co.uk/1425/68/products/artseq-ribosome-profiling-kits/

http://www.epibio.com/applications/rna-sequencing/ribosome-profiling/artseq-ribosome-profiling-kits

Rasha Al-attar

Thanks