I‘m an undergrad student working with ABC and MATE transporters and i was reading this paper http://www.plantcell.org/content/31/11/2789 bc they were using yeast microsomes for transporter assays and i was wondering how that works exactly? at one point they mention „yeast microsomes overexpressing transporter..“ and i didnt even know microsomes were able of transcription and translation. to my knowledge microsomes were just vesicles formed by the ER membrane and random cell compartments that form after a cell is broken up and not somewhat functioning „cells“. i couldn‘t find anything online on what yeast microsomes are exactly or what they‘re capable of so i‘m hoping someone on here knows anything or has worked with them before!
Hi there,
Microsomes are purified vesicules originating from ER and Golgi. The expression „yeast microsomes overexpressing transporter..“ is abusive. Microsomes may be used as substrate in translation/translocation assays. This is possible if you provide mRNA and full translation machinery and substrates. For membrane and secreted proteins, you may observe enrichment of proteins in microsome membrane and microsome lumen respectively. So „yeast microsomes overexpressing transporter..“ is the result of this assay with mRNA encoding for the transporter.
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