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Fermentation medium is the substrate which a microorganism biotransforms by means of its biocatalysis mechanism to produce a new biochemical product while a yeast suspension is the fermentation broth on which the microorganism grow while the fermentation process proceeds. Preparing a yeast suspension for instances yeast extract, you can obtain information from a purchase pack of the yeast extract. There you are told what quantity to weigh and dissolve in knowed quantity of water before sterilization using an autoclave. Besides, for a fermentation medium preparation, the concentration of the substrate is first determine while it is dilute to your choice of concentration using the serial dilution formular of C1V1= C2V2.