As we know DNS is an internationally acceptable method for xylanase enyzme activity assay. It has been use as a method to calculate the enzyme activity of xylanase by measuring the reducing sugar release by the reaction. IUPAC has also recognize DNS method for xylanase activity in a paper publish in 1992. In the same year Bailey also recommend DNS assay for checking the xylanase enzyme activity.
But in recent years, the effieciency of DNS method for xylanase enzyme activity have been doubts and questioned through recent research. For example a study by Alexander V. Gusakov et. all (2011) and Barry V. McClearly (2015).
My question is can the DNS method still be used to determine the xylanase enzyme activity?.
If the answer is no, what is the status of the recent research on xylanase using DNS as a method? is it still acceptable since it have been already widely used or it is unacceptable?
Thank you Dr. Luong for your feedback. I've go through the article before.
I am hoping if any other opinion or study that support the usage of DNS as a method to calculate xylanase activity, I would like to know.
Is a myth of infallibility of the scientific method?
Science has limitations ?. If it does, what are they?
We liberating art to make science?
- Badges
- Science topic