There are variable methods for determination the composition of the culture filtrate of Trichoderma harzianum, I need, if possible, more reliable and accurate methods for this purpose. Thank you
Many thanks to Mojegan Kowsari, Ali Abdel-Hadi Alsudani and Ali R Mulakhudair for valuable recommendations. I mean how to optimize the extraction procedures for raw material, extraction time and solvent concentration. As the optimization for processing parameter for culture filtrate of Trichoderma harzianum are quit necessary before analysis GC-MS. Thanks again
actually, I extract many chemical compounds from culture filtrate of Trichoderma, hormones, organic acids and enzymes. but each one has a different procedure to identify and estimation. so I think if you used GC-mas you will discover more compounds -according to the device library- .