Would the reducing agent TCEP remove the Npys group and the reaction with iodoacetyl group could still proceed? Or it will just not react?
Reducing with TCEP changed the colour of the Npys protected Cys containing peptide solution to strong yellow from slightly yellow. This indicates that the Npys came off.
No, it will not react since you need a free S- for the reaction.
yes, yes and it will.
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