Would alcohol administration affect GAPDH expression at the protein level?

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Sang Ho Lee

Hello, Daniel.

People suggested more robust house-keeping gene, beta-actin than GAPDH. Alcohol may influence carbohydrate metabolism a lot. Furthermore, in cells affected highly by any treatment, GAPDH is reported to translocate into the nucleus to be involved in cell death process. You would lose nuclear GAPDH in these early-mid stages of cell death during cell lysis.

Best wishes.

Han Zhang

I agree with Sang. Why don't you try another loading control? GAPDH can affect metabolic process, and so does alcohol administration.

Daniel Schwartz

Thank you both so much.