How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

How do i handle Ti and/or Aluminium powder. Since in the instruction on the container is written that CATCHES FIRE SPONTANEOUSLY IF EXPOSED TO AIR ?

i am working on max phase preparation......i am a newly joined PhD scholar. I wanted to make MAX phase of Ti3AlC2 , in most of the literature they are using the metal powder of Ti and Al for the...

23 December 2020 7,122 8 View

Is the assumption about Delaunay edge true?

01 July 2020 9,998 2 View

Lysis Buffer for RNA extraction?

Dear all, I am working on a magnetic beads based RNA extraction project. Presently, I managed to come up with a lysis to extract RNA from blood samples. However, my PCR results show inhibitory...

06 April 2020 9,953 3 View

Homing Receptors for tumor infiltrating T cells?

I am researching how different culture and expansion conditions contribute to a T cell's infiltration of a solid tumor. Was wondering what the best surface / intracellular receptors or ligands are...

26 June 2019 5,072 0 View

What is the source of basic 'hydroxyl' sites on gamma-alumina surface? Can hydroxyl ions form on its surface with only water present?

Consider an initially non-hydrated gamma-alumina surface. I pass water over the surface hoping it to dissociatively adsorb on the surface giving me hydroxyl radicals adsorbed on alumina surface....

31 March 2019 8,615 2 View

Does any one have a detailed protocol for isolation of Glucocerebrosidase from rat brain, please don't mind to share?

I am working on Gaucher"s disease, in this connection I would like to isolate GCase enzyme from rat brain. so please share the protocol if available. This isolation process should not include HPLC.

27 March 2019 2,591 1 View

Why the cylindrical tube at the corner of the square are not following the curve while performing the 'sweep' action in COMSOL Multiphysics?

I am completely new in working with COMSOL Multiphysics. I am trying to draw a cylindrical shaped wire wound around the square block(as seen in the attached picture). The cylindrical wire is...

10 March 2019 3,104 3 View

How can i coat activated alumina or activated carbon on metal such as copper? What's the process?

How can i coat activated alumina or activated carbon on metal such as copper? What's the exact process to do? Is it feasible?

31 October 2018 6,171 2 View

How do I prevent loss of RNA during DNase I treatment of low amounts of RNA?

I am collecting rare cell types from the nematode C. elegant by FACS for RNA extraction and subsequent sequencing. For some populations we get 5,000 - 10,000 cells. We sort directly into TRIzol...

28 August 2018 6,205 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View