Hi, we are working on stool-metagenomics based on V3V4 and ITS2 amplicon sequencing. We would like to analyse data in a work station by using QIIME. Can someone help me with the best configuration (type of processor, RAM, hard disk storage) of work station that is required for analysing this NGS data (preferably for the data that is generated on an Illumina-MISeq platform; assuming for each sample nearly 2 GB of data is generated; and we need to analyse a maximum of about 120 such samples at a time).
Hi. I had been working with about 25 millions of sequences from MiSeq in QIIME. My computer has 16Gb-RAM and 8 processors and it was enough for make all the analysis. And if you have a hard-disk of 1Tb you have so more than you will need.
Good luck with QIIME, and try to install this on the computer (Linux) because QIIME Virtual Box could limit the RAM you can use,
Thank you Jose Francisco. What does "8 processors" mean? could you please explain me.
I have the Processor Intel® Core™ i7-6700 CPU @ 3.40GHz × 8, In this web (http://ark.intel.com/products/88196/Intel-Core-i7-6700-Processor-8M-Cache-up-to-4_00-GHz) you can check the characteristics. It have 4 cores, and 8 threads, it is good because in a lot of software you can use more than one thread, that makes it faster. And it is so important to have a 64bit operation system, because with 32bit you can't use all the RAM of the computer.
Sorry if I have not explained so well... but basically more threads is more processing powerfull and less time to finish the processing.
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