Hi,
Does anyone have experience with preparing cDNA libraries from small RNA, for next-generation sequencing?
If so, has anyone tried doing it in PCR plates instead of strip tubes? I have a large amount of libraries so I believe it is better this way. My only concern is from cross-contamination between neighboring samples?
Thanks
Iddo
Do this routinely. Aliquot the RNA either the first step or the last step in the protocol. Also I use MS Excel when doing this since very often my RNA volume and consequently water volume across samples will be different which means I need to keep track of what I am adding and where I am adding. Customize the Excel toolbar and add the speak upon enter button to the top. Select the column of RNA volumes that you are going to add and press enter for the first sample. This way you can double check the well number and the volume each time without losing track. I am writing a small tech note on it shortly and will post a link when done.
Dear Abhijeet,
Thanks.
What type of plate are you using? (ie manufacturer, item # etc.)
Iddo
We typically purchase qPCR plates from Agilent but I guess any good quality semi-skirted / skirted plate would work.
AB
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