Validating NGS data with regular Taqman or advanced Taqman miRNA assay?

Hi, I wish to validate NGS data of miRNA sequencing from human plasma samples. The obvious way to do it is of course Taqman, but there are two different assays: the 'traditional' one in which...

31 December 2018 6,963 0 View

How to find reference genes for qPCR from RNA-seq data?

Hi everyone, I did RNA-seq for small RNA, and now I want to confirm the results with qPCR. What is the best way to find reference miRNA genes from the NGS data, for the normalization of the...

31 December 2018 712 4 View

Discrepancy between DESeq2 data and raw data?

Hi, i performed NGS for small RNA and ran DESeq on the data. there is one miRNA that is upregulated by 2-fold in the patients compared to healthy controls. However, when i look at the raw...

06 July 2018 3,398 3 View

Protocol for plasma preparation?

Hi, I need to isolate plasma from blood for RNA extraction afterwards. Does it have to be in a cold centrifuge? Do you need in general to isolate plasma in a refirigerated centrifuge or is it...

04 May 2018 5,998 5 View

Doing more PCR cycles to get higher concentrations of libraries?

Hi, I am preparing small RNA libraries for NGS, using the QIAseq kit of Qiagen Usually I get Canadian concentrations of around 1-10 ng/ul, but in the last batch all of the libraries were

04 May 2018 1,975 5 View

Is "camel hump" in tape station a sign for adapter dimer?

09 October 2017 2,433 1 View

Perfusion or post-fixation?

Hi, I have brains that express GFP under a promoter of an endogenous protein so they actually express a fusion protein. I want to visualize GFP and thus know where the endogenous protein expresses...

04 May 2017 3,628 6 View

PCR plates or PCR strips for library prep?

Hello, If I use a PCR plate for library prep and I need the products after each step in the protocol (in my case it's polyadenylation, RT and PCR), is it safe to open the plastic foil that covers...

03 April 2017 9,111 3 View

Does anyone know of a method to measure enoxacin in plasma?

Hi, Does anyone have an experience with measuring levels of enoxacin which is a fluoroquinolone antibiotics, in the plasma? Thanks Iddo

01 January 1970 8,690 3 View

Working with PCR plates for large-scale preparation of cDNA libraries for sequencing?

Hi, Does anyone have experience with preparing cDNA libraries from small RNA, for next-generation sequencing? If so, has anyone tried doing it in PCR plates instead of strip tubes? I have a large...

01 January 1970 9,534 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View