Hi, I am looking for a pipeline to analyze bioinformatically Fastq files from small RNA sequencing.
Can anyone recommend something that is straightforward? Do I need to store the file on a special server?
Thanks
Iddo
Herein I list 4 tools most used small RNA-Seq:
1) Based on Encode
https://www.encodeproject.org/pipelines/ENCPL337CSA/
2) iSmaRT
http://bioinformatics.oxfordjournals.org/content/early/2017/01/05/bioinformatics.btw734.abstract
3) iMir
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-14-362
4) miRDeep2
https://www.mdc-berlin.de/8551903/en/
Hope this helps you!
Please visit these pages:
http://sfg.stanford.edu/quality.html
https://wikis.utexas.edu/display/bioiteam/Evaluating+your+raw+sequencing+data
Thank you for such interesting question and useful answers, sorry I do not have an answer but I really found it very intriguing.
Although I am late but I think if I share my book chapter, it could be helpful.
https://link.springer.com/chapter/10.1007/978-94-024-1045-7_10
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