Working with Methycellulose for Plaque Assays?

30 January 2014 1 9K Report

I'm using methylcellulose as an overlay for PA (before I always used Agarose) does anyone have suggestions of

#1 how to get this stuff dissolved

#2 be able to pipette so you can make a 1:1 solution with MEM

I tried today to pipette my 2% solution of methylcellulose and it was so viscous that it was almost impossible to pipette and be accurate.

Constanza Pamela Martinez-Valdebenito

Hi julie:

What I usually do is to autoclaved the powder (exactly and then under the hood add to a DMEM or MEM. And put in the 4°C with a shaker for 1 night or 1 day, even the weekend and then you have your methylcellulose ready to add FS and antibiotic and ready tu use.

Hope this can help you

Constanza

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