Without the equipment to culture the THP1, how can I effectively infect this suspension cell?

26 November 2013 1 7K Report

I have the second generation and the third generation lenti-virus package plasmid.

Please explain what you mean by "without the equipment". If you do not have cell culture hood or some way to work aseptically, you can't culture mammalian cell lines such as THP-1. Otherwise, lenti transduction do not require special equipment, besides what is already needed for mammalian cell culture.

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