Dear colleagues, and Senior Scientists,
I am setting up a cell culture laboratory, but no funding for an inverted microscope now, and viewing my culture for growth and confluence will be a challenge. I am of the view that a simple set-up using hand lens and/or camera can help me view the progress of my cells. I think this is cheap, and can help me start my lab while I work towards getting a standard inverted microscope. Has anyone successfully used such an improvised technique? A guide on how to set-up these devices to view cultures or any alternatives will be very much appreciated. Thanks in anticipation.
Dear Williams,
That depends on exactly what you want to watch. If you want to look at individual cells you will not be able to improvise anything without spending the equivalent of what you would spend buying a microscope. On the other hand, if you want to evaluate cell cultures by size and or edge growing is possible. But anyway you will have to invest money with camera, lenses, acquisition boards and evaluation software. Unfortunately it is not possible to do research in this area at a professional level without a minimum investment. It seems to me that the inverted microscope may be cheaper than a lot of discrete components which as a rule will not reproduce the same results as you want. I'm sorry but this is the reality of the situation...
Antonio
Thanks so much Antonio Oliveira for your advice. I have gained some more insight into my earlier thought.
Justin R Croft, thank you for your suggestion, I following the link to explore furthers.
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