Will the human insulin act upon the L6 cell lines (derived from rat skeletal muscle)?

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Can anyone explain how to calculate encapsulation efficiency(Plant extract encapsulated alginate nano particles) using total polyphenol content?

Please suggest me if there are any reference materials for this topic. Thank You

30 December 2020 3,799 2 View

Electric field inside a biased N-type uniformly doped semiconductor?

What does the profile of electric field look like in a uniformly doped N-type semiconductor when an external voltage of 'V' volt is applied? Please provide any reference material if possible.

06 November 2020 1,987 8 View

Plane stress or plane strain for Railway Ballast system ?

Dear All, I am working on a wooden rail sleeper analysis. Using the Druker Prager model for Ballast and soils, Initially, I assumed the 2D Behavior of RailSleeper and Rails was Plane Stress and...

04 October 2020 5,459 8 View

What should be Optimum Review duration for a research/review article for any [unpaid/freely publishing]journal till acceptance?

Dear Research Gate Members, We all have experience regarding publications of research/review articles in various journals. Some of the journals review the articles in 4-6 weeks and some takes...

01 October 2020 7,201 1 View

How to write an impressive review paper in the field of Industrial effluent treatments?

I am currently working on collecting literature based on the available treatment technologies for industrial effluents. There are vast variety of methods physical, biological and Advanced...

23 September 2020 2,314 4 View

Fibrous rock with triclinic symmetry?

I am working on wave propagation in fiber reinforced material. Please provide me an example of rock-type which is consisting property of fiber and exhibiting triclinic symmetry with its elastic...

20 September 2020 9,874 1 View

How to pursue modelling and map Traditional Knowledge?

I was wondering is it possible to map Traditional Knowledge (TK) of a bigger region (say staus of TK) on the basis of sampling in a smaller region through Arc GIS and modelling? Say we sampled in...

13 August 2020 308 3 View

Pressure behavior from CFD simulation and testing?

Hello, I am simulating gas flow from a high-pressure (700 bar) to a low-pressure container as shown in the attached figure. The red curve shows the pressure curve obtained from the testing, while...

04 July 2020 2,077 2 View

Ansys Fluent remeshing on hex mesh?

Hello, I went through ansys fluent dynamic meshing guide. It says remeshing can be done only on tetrahedral mesh. I still tried to implement it on hexahedralmesh. It works well. It is bit...

03 May 2020 2,677 4 View

How AI and Deep Learning can help to Screening Corona virus (COVID-19) ???

There are many Articles out there related with Deep Learning with corona virus, For Example : "deep learning algorithm using CT images to screen for Corona Virus Disease (COVID-19), Deep Learning...

12 March 2020 3,780 9 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View

What is the best way to minimize/prevent the effects of rigor mortis in excised mouse muscle?

I'm planning to excise mouse muscle to use for ex vivo mechanical testing. What is the best way to prevent the stiffening effects of rigor mortis from confounding results if I cannot isolate the...

30 January 2021 1,641 2 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View