I am making retrovirus with pBABE-GFP and ECO packaging vectors in the clonetech packaging cell line GP2-293. I noticed that my host cells are not green. Is this ok?
Have you done PCR and RT-PCR to test for transfection and expression? I would start from there.
What do you mean by "be green"? They should exhibit green fluorescence when excited by blue or UV light. If you can't detect pBABE-GFP expression by fluorescence microscopy, something is certainly wrong.
If you mean, should the cells look green by eye, they can, but mileage can vary. Not in a cultured mono-layer. It's too thin, and phenol red in the medium gets in the way.
If you detach them and spin them down, cell pellets always appear yellowish-green. When you express GFP, you can often see an extra-green hue on top of that, but you need a trained eye, or better a control, to be sure. So by eye I wouldn't worry about it.
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