Peter Yang

2 Questions 28 Answers 0 Followers

Questions related from Peter Yang

Any advice with my His-tag protein purification with talon affinity column giving lower yield at larger sample volume?

Hi, We have encountered something strange. Our group is purifying an N-tagged 6xhis protein with Talon 5ml prepackaged column. When we first tried with 2ml sample volume, everything worked great....

07 July 2015 663 3 View

Can yeast mannan oligosaccharide enhance hemagglutination activity of a lectin?

We are trying to express a lectin in E.coli, which is specific to high mannose glycans. Using akta system and size exclusion column (Sephacryl s-200), we isolated protein though at a pretty low...

05 May 2015 4,670 1 View