Will NanoDrop be an accurate way to measure total protein content from the supernatant of a homogenized ligament sample?

More Amjad Ramahi's questions See All

How many grams or milligrams of Artemia should be given to 6 dpf larvae?

Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?

13 June 2024 389 0 View

Tensile Strength of Cement Mortar ?

Can anybody recommend the tensile strength of cement mortar? Can ASTM C307 be used to test for mortar without fibers?

03 June 2024 7,550 1 View

How measure tolerance factor of BaSrCaTiO3 perovskite?

for ABO3 if only A & B then we used t=rA+Ro/sqrt2(rB+rO) kindly explain for BaSrCaTiO3 ?

31 May 2024 2,036 0 View

what is the relation of macrostrain with particle size?

some data shows direct and some inverse relation? kindly share any authentic paper please.

07 May 2024 6,657 4 View

I need method to prepare silymarin cream ? if available ... precise method with details ?

I need to prepare silymarin cream , I am facing some difficulty with that

06 May 2024 3,614 0 View

Mechanism of copolymer?

Kindlly provide links or literature about the mechanism of free radical copolymerization of styrene and methacrlate, kinetics and rate equation

27 April 2024 404 2 View

What could be the reasons of protein not expressing in Bacillus Subtilis and Bt??

I and using vectors with autoinducibe promoters(Pylb and cyt1). I have performed SDS of 24, 48, 72, 96 and 120 hours. No expression is found in soluble or insoluble form.

14 March 2024 8,752 0 View

3T3-L1 cells are not differentiating after 5 days of induction. What could be the problem?

I induced the preadipocyte after they reached 100% confluency. Then, I treated them with differentiation medium (hg DMEM 10% FBS, 1X P/S, IBMX 0.5mM, Dex 1uM, and Insulin 10 ug/ml) for 2 days....

18 February 2024 8,219 0 View

Deepsoil/Shake 91 multiple motions addtion?

Is there any way to add multiple motion files in a single go to a deepsoil software or to run Shake91 in loop form

30 October 2023 8,858 0 View

Anyone helps me explain textural features in apatite?

The bright zone is high in REEs up to 4% and other zones are depleted in REEs. Can we say overgrowth? But they are enclosed in apatite middle.

25 September 2023 4,667 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Is there a method to measure the concentration of Cs, Sr, Co using spectrophotometer?

it's important to know that we are using the inactive elements of Cs, Sr, Co

25 July 2024 1,553 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

Subhash C. Juneja

I doubt it will measure homogenized sample. Sample need to be full dissolved, Homogenization is first step of protein extraction, you may need to extract protein and dissolve in water fully. Than nano drop may read your sample.

Let us hear from other protein biologists,

Best

Amjad Ramahi

Thank you Subhash.