Will Macroconidia of Dermatophytes be visible on a tape impression smear taken from a dog?

Ranjani Rajasekaran @Ranjani-Rajasekaran

04 February 2025 0 3K Report

A dog was brought with a history of dandruff and itching. Tape impression smear was taken from the dorsum region. Upon staining it with Lactophenol Cotton Blue stain, I found Macroconidia. But I have doubts about these.

Is this a Macroconidia? - photo attached

If yes, can you help me identify the Dermatophyte involved? Microsporum canis or Microsporum gypseum? or something else?

Can identification of Macroconidia on a tape impression smear be confirmed as a positive case for dermatophyte infection? or will it be a non-parasitic stage?

When I tried to culture it, fast growing fungi was observed when incubated at 37 degree celcius. I am yet to get results of the plate that is incubated at 25 degree Celsius.

Are there any other advise on how to proceed with this?

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