When we dissolve the commercial standard of GALANTAMINE HYDROBROMIDE in the water or any solvent, Will get separated into Galanatamine and HBr, or will it be bound together?
I am performing HPLC of my plant extract, to find out the GALANATAMINE quantity, for that, I am using "Galantamine Hydrobromide" of SIGMA as a reference standard. When we run an HPLC of Galantamine-HBr alone we are getting a Sigle prominent peak, previous reports suggesting that the peak is Galantamine. Here my question is, in the HPLC graph the peak is GALANTAMINE or Galantamine-HBr?
if it's Galantamine where is the HBr peak, and if it is Galantamine-HBr How can we take it as a reference for quantifying galantamine?
Badhepuri Mahesh Kumar When we add solvents with galantamine, counterion exchanges, hence replacement of bromide ion (in general lactate or gluconate in case of live system) will occur to promote solubility of galantamine.
Thank You Noel W Davies Sir for your valuable time
Here the HPLC conditions are
Mobile phase: 1:10 acetonitrile : 0.4% KH2PO4 (in water)
sample of Injection: 10 uL
Runtime: 15 min for Standard
C18 Column
Shimadzu HPLC machine
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