I am testing an enzyme, arylacetamide deacetylase's activity toward different substrates. I am not sure whether the enzyme I am using, which is expressed in the Sf21 cell, will have the same deacetylase activity as the enzyme expressed in the 293T cell?
If post-translational modifications (PTMs), such as phosphorylation or glycosylation occur, then they may be different between the insect and mammalian cells. If those modifications affect activity of the enzyme, then the enzyme's activity could be different when expressed in the different types of cells. If you have samples of the enzyme from both expression systems, you can do the comparison. You can also have an intact protein mass spectrum taken of each one to find out its molecular weight, which would tell you about the PTMs.
Thanks, I will compare the activity of the enzyme expressed in different cells.
I would not expect that , except if there is a theory to explain possible changes it may undergo in different host cells, but coming from a different host may influence its extraction/purification ...host cell added impurity(ies)/ extraction protocols may affect the final activity of the enzyme.
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