Hi everyone, i'm having problems with the MTT assay i'm performing, since two weeks.
I'm performing viral infection on Beas2B cells and a dose response curve of compounds with citotoxicity in parallel.
When i add MTT on the infected cells the wells get completely purple, while on the non-infected (citotoxicity) I see normal metabolism inside the cells.
What could be the reason? Do any of you have experimented something like this?
Hi Annalaura!
I haven't worked with infected cells, but I'm thinking of two things that could possibly cause this effect:
1. Are you sure you don't have any bacterial or other contamination in the infected cells?
2. Do you add any compounds to the medium with infected cells that are not present in the medium with normal cells? Maybe there are compounds that are able to reduce MTT?
Zuzanna Koziara Thank you for your answer.
1. the citotoxicity and the viral infection were performed in parallel, i thought about a possible contamination but i should have seen the very same result in the non infected cells (unless the tube where i made the viral dilution was contaminated).
2. the same compounds' dilution were use for both the experiments.
The experiment was performed in one plate, half was infected, the other half wasn't.
The cells are BEAS2B and are at p22, i thought they could be too old and went under oxidative stress.
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