How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

Hi, could anyone suggest any mammalian expression plasmid that has a GFP as a selection marker?

I am looking for a plasmid that can express my gene of interest without GFP fusion. But I want its selection marker like neomycin replaced by GFP or any other fluorescent protein so that I can...

29 October 2020 2,247 3 View

Predict charge on a compound at different pH?

Dear fellow scientists, Do you know of a software or a resource base which can predict the charge on the analyte at different pH conditions? Thank you, Abhishek P.S. I am new to HPLC, even a...

19 October 2020 8,605 3 View

How to define Darcy's flow for pore pressure cohesive element in Abaqus?

Hi everyone, I am trying to define a Darcy's flow for pore pressure cohesive element in Abaqus. When I give permeability to the elements, the output of tangential flow rate (GFVR) is always shown...

11 October 2020 1,195 3 View

What is the model equation between resistivity and temperature of a extrinsic semiconductor?

Can anyone Tell me what is the model relation between resistivity and the temperature for a extrinsic semiconductor. Kindly provide with summarized clarification & model equation between...

30 August 2020 6,182 11 View

What is timely Supply chain management issues to be researched as a result of the COVID 19 pandemic?

Dear Research Scholars, Would you please share your opinion on "What is timely Supply chain management issues to be researched as a result of the COVID 19 pandemic?" Thanks Shuvro Sen

22 July 2020 6,995 15 View

Does anyone have a case study of kick/influx and fluid losses during drilling, while MPD (managed pressure drilling) system is active?

Looking for a case study where severe influx and loss events were experienced even when a MPD system is installed and active. Interested in the sequence of events and mitigation steps. A real well...

12 June 2020 8,922 3 View

My SDS gel takes 4-5 hours to run completely. Can someone please help me understand what might be going wrong?

I am performing DNA-Protein Binding assay using Dyna beads. In order to check the bound protein i have to run SDS PAGE. the problem I am facing is my gel takes 4-5 hours to run completely. I have...

09 June 2020 7,358 1 View

Large sample sizes and parametric or non-parametric tests?

I have been reading several sources mentioning that when you have large enough sample sizes (some say 20, others above 200?) then parametric tests, such as t-test, are robust against non-normality...

07 June 2020 3,354 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View