We are working on RT_LAMP assay with Warmstart colorimetric 2X master mix E1804. We have a LAMP reaction and it is a ladder-like pattern on an agarose gel. The Tt(time of threshold) is about 19 min.

We guess the problem is related to Buffer ion concentration or other ingredients such as dNTP or unit of the enzyme(such as Bst). Is there anyone know these ingredient concentrations?

The other thing that we think may have effects on this result is the power of our primers.

Also, we have LAMP reaction with positive RNA (from medical Laboratory) until 10ng/ul, we may have no strong set of primers that can produce good turbidity or color change.

I wish you say your opinion according to your experience.