I checked the literature in detail to compare different cell viability, cytotoxicity studies to check the biocompatibility of biomaterials. In some articles, they use the 'leachable-conditioned medium' method to check the biocompatibility. For this purpose first, they incubate biomaterials in cell culture medium without FBS after that they use this 'conditioned medium' with FBS in cell seeding. I am wondering in this first step why they don't add FBS directly to cell culture media and is it important for this procedure. I also saw different methods including FBS.
I am waiting for your response. Thanks:))
FBS contains cell adhesion proteins as well as growth factors. Conditioned medium contains secreted cell adhesion and growth factor products produced by cells in culture. Added FBS to conditioned medium would cover up or inhibit effects of the conditioned medium.
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